Articles - Organotechnie

Ecovadis validates corporate adherence to 21 recognized CSR criteria which follow verifiable international CSR standards (the Global Compact Principles, the International Labour Organization conventions, the Global Reporting Initiative standard, the ISO 2600).

These 21 CSR criteria are divided into 4 fundamental themes: Environment, Social and Human Rights, Ethics and Responsible Purchasing.

In order to continuously improve our CSR performance, we have recently developed a responsible purchasing charter and a code of ethics and business conduct. These actions have led to a 50% increase in our rating in the Responsible Purchasing theme.
In addition, we have completed our first carbon footprint and have put in place the key elements of a GHG management system as well as an interim commitment, actions and reporting capabilities for decarbonization such as the purchase of renewable energy.

For our latest rating, carried out on September 11, 2024, we obtained a silver medal and achieved a score of 68/100, which places Organotechnie in the top 15% worldwide.

Organotechnie R&D Dept. conducted a study to evaluate the performance of plant-derived Hydrolysates for protein production in HEK293 cell cultures. The research compared various hydrolysate sources against Tryptone N1, the standard supplement used post transfection in mammalian cell culture protocols.

The study used HEK293-6E cells with pTT28 vector to express His-tagged human interleukin-4 (hIL-4) in serum-free media supplemented with 0.5% Hydrolysate. Protein quantification was performed using Western blot analysis with anti-His tag antibodies at 120 hours post-transfection.

Results showed that three plant-based Hydrolysates produced higher yields than the control: C-CELL P112 (pea-derived) achieved 339% of control levels and C-CELL G115 (guar-derived) attained 324%. These represent approximately 150% increases over standard Tryptone N1 performance.

Plant-based Hydrolysate offer several practical advantages including absence of animal-origin contaminants, simplified downstream processing, and reduced regulatory concerns for therapeutic applications. The study maintained consistent culture conditions and production processes across all test conditions, with IL-4 protein being predominantly secreted into the culture medium.

These findings indicate that C-CELL plant-derived Hydrolysate can serve as efficient supplements in mammalian protein expression systems.

September 2025

More details in the poster here: Poster HEK293.pdf

The study, conducted by Shabir et al. at the Institute of Parasitology, evaluated peptone supplementation during expression of a 34 kDa Echinococcus granulosus tegumental protein. Using quantitative Western blotting with fluorescent antibody detection, the team compared Typtone N1 with Pea C-Cell P112 and Broad Bean C-Cell F120 peptones across three independent experiments.

Results showed no significant difference in normalized protein expression between the three peptones, with all three significantly outperforming no-peptone controls (p<0.01). Cell viability and density profiles remained similar across all peptone treatments throughout the culture period from 24 to 120 hours post-transfection.

These findings address current concerns regarding animal-derived products, including disease transmission risk, batch variability, and regulatory constraints. The plant-based alternatives eliminate potential mycoplasma and viral contamination sources while supporting sustainable bioprocessing practices.

The study suggests plant-derived peptones represent a viable alternative to TN1 for recombinant protein production in mammalian cell systems, with potential implications for both research-scale and commercial biomanufacturing applications.

October 2025

More info on the poster : HEK293- Protein expression ORGANOTECHNIE

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